FIG. 1.

Genomic analysis of the B. bovis msa-1 locus. (A) Map of the Mo7 msa-1 locus based on sequencing of a 5.520-kb fragment contained in the msa-1-Sau3A genomic clone. The four ORFs designated by patterned boxes correspond to the following genes: orf-1, low homology to polyketide synthase gene from Streptomyces spp.; orf-2, msa-1; orf-3, significant homology to N-methylaspartate receptor protein gene; orf-4, low homology to a hypothetical gene of C. elegans. Arrows indicate the orientation of the coding DNA strand. Primer sites (orf1-f, orf1-r, msa1-f, msa1-r, orf3-f, orf3-r, orf4-f, and orf4-r) are designated above and below the map of the fragment, with primer orientation indicated by arrows. EcoRI sites and the size of the corresponding EcoRI fragment (2.4 kb) are shown below the map. Intergenic regions (white boxes) and the introns in orf-3 and orf-4 (black boxes) are indicated. (B) Transcriptional analysis of the B. bovis msa-1 locus represented by an ethidium bromide-stained agarose gel of amplicons specific for each of the ORFs encoded in clone msa-1-Sau3A. For each ORF, three lanes are depicted. Lane 1 represents amplification of Mo7 mRNA with reverse transcriptase, lane 2 is a no-reverse transcriptase control, and lane 3 represents amplification of DNA. Size markers are shown at the right.