Genomic analysis of the B. bovis
msa-1 locus. (A) Map of the Mo7 msa-1 locus based on
sequencing of a 5.520-kb fragment contained in the
msa-1-Sau3A genomic clone. The four ORFs designated by
patterned boxes correspond to the following genes: orf-1,
low homology to polyketide synthase gene from Streptomyces
spp.; orf-2, msa-1; orf-3,
significant homology to N-methylaspartate receptor protein gene;
orf-4, low homology to a hypothetical gene of C.
elegans. Arrows indicate the orientation of the coding DNA strand.
Primer sites (orf1-f, orf1-r, msa1-f, msa1-r, orf3-f, orf3-r, orf4-f,
and orf4-r) are designated above and below the map of the
fragment, with primer orientation indicated by arrows.
EcoRI sites and the size of the corresponding
EcoRI fragment (2.4 kb) are shown below the map. Intergenic
regions (white boxes) and the introns in orf-3 and
orf-4 (black boxes) are indicated. (B) Transcriptional
analysis of the B. bovis msa-1 locus represented by an
ethidium bromide-stained agarose gel of amplicons specific for
each of the ORFs encoded in clone msa-1-Sau3A. For each ORF,
three lanes are depicted. Lane 1 represents amplification of Mo7 mRNA
with reverse transcriptase, lane 2 is a no-reverse transcriptase
control, and lane 3 represents amplification of DNA. Size markers are
shown at the right.