FIG. 3.

(A) Comparison of the predicted MSA-1 amino acid sequences from the Mexico Mo7 strain and the Argentina strains R1A and S2P. Amino acid substitutions, insertions, and deletions are indicated by a white background. Areas of amino acid identity have a black background, while conservative amino acid substitutions are enclosed in gray boxes. Dots indicate deletions. The number of amino acids in each sequence is shown on the right. (B) Cross-reactivity of antibodies against MSA-1 from the Argentina strains R1A and S2P. Serum raised against rMSA-1 from strain R1A or S2P was reacted in immunoblots with control URBC (n-RBC) or erythrocytes infected with the R1A strain of B. bovis. The position of the 45-kDa R1A MSA-1 molecule is indicated on the right.