FIG. 3.

S. carnosus clones expressing FnBPs dose dependently bind to immobilized and soluble fibronectin. (A) Adherence of S. carnosus strain TM300 transformants to immobilized fibronectin (Fn). PMMA coverslips were precoated with 0.5 mg of gelatin per ml in order to optimize fibronectin adsorption (see Materials and Methods). Results are means and SEMs for four independent experiments run in duplicate. (B) Binding of soluble FITC-labeled fibronectin (Fn-FITC) (5 μg/ml) by S. carnosus strain TM300 transformants. Results are means and SEMs for four independent experiments run in duplicate.