Figure 6.

Sequencing on Flongle flow cells detected variant breakpoints in a shallow Cas9-mediated long-read approach. (a,b) Sequencing data from two dual-cut excision approaches sequenced on a Flongle flow cell. (a) Detection of the two-exon deletion (*, red star) of exons 12 and 13 in CCM1 (Figure 1) with a sequencing coverage of 6×. (b) Re-sequencing of a heterozygous two-exon deletion (*, red star) of exons 3 and 4 in CCM2 with a Flongle flow cell yielded a sequencing coverage of 4×. No wild-type reads were generated. Read data were inspected in IGV [23]. The Locus Reference Genomic (LRG) transcripts are shown (a,b).