Figure 5.

Comparison of effect of different Asf1 proteins/peptides (A) on CK2 activities from human (B) and yeast (C). Phosphorylation activity of CK2α (black bars), human CK2α₂β₂ or yeast CK2α₂ββ’ (light grey bars), and CK2α’ (dark grey bars) and human CK2α’₂β₂ or yeast CK2α’₂ββ’ (white bars), was measured in the presence of Asf1 or its fragments. The relative activity is given in reference to CK2 incubated with trypsin and STI as control. The experiment was carried out in the presence of 10 μM P2B and 20 μM ATP. * and ** indicate significant difference compared to the controls (p < 0.05, p < 0.01).