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. 2022 Dec 13;23(24):15834. doi: 10.3390/ijms232415834

Figure 2.

Figure 2

Subcellular localization of C488 mutant spike protein in Vero cells. Wild-type (WT) and C488A mutant spike-expressing plasmids were expressed in Vero cells for 2 days, and their subcellular co-localization with (A) calnexin, (B) ERGIC53, and (C) GM130 were analyzed by confocal microscopy. WT and C488A mutant spikes (green signals) were probed with anti-spike (1A9) antibodies and subcellular markers (red signals) used were: calnexin, ERGIC53, and GM130 as ER, ERGIC, and Golgi markers, respectively. A co-localized yellow signal is visualized in the merged images (lower panels).