Table 1.
Plasmids used in this study.
| Plasmid Names | Remarks, Protein Sequence and Mutation |
|---|---|
| pEGFP-C1 | BD Biotech Clontech |
| pcDNA3.1–spike/WT | Wuhan Hu-1 spike, YP_009724390.1 |
| pcDNA3.1–M | Wuhan Hu M, YP_009724393 |
| pcDNA3.1 SARS-CoV-2 N | pcDNA3.1 SARS-CoV-2 N was a gift from Jeremy Luban (Addgene plasmid # 158079; http://n2t.net/addgene:158079, accessed on 15 November 2022; RRID:Addgene_158079) [29] |
| pcDNA3.1–spike/D614G | D614G |
| pcDNA3.1–spike/C488A | C488A |
| pcDNA3.1–spike/C488R | C488R |
| pcDNA3.1-spike/C488F | C488F |
| pcDNA3.1–spike/Alpha | H69/V70-del, Y144-del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H |
| pcDNA3.1–spike/Delta | L5F, T19R, E156G, F157/R158-del, L452R, T478K, D614G, P681R, D950N |
| pcDNA3.1–spike/Omicron (BA.1) | A67V, H69/V70-del, T95I, G142D, del143–145, ins214EPE, NL211–212I, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F |
| pcDNA3.1-hACE2-DYK | GenScript, Cat No:OHu20260D |