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. 2022 Dec 12;23(24):15742. doi: 10.3390/ijms232415742

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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SacsJ directly interacts with NFL in vitro. (A) GST pulldown assay of soluble recombinant NFL with GST-SacsJ, or GST as control. (A) Ponceau red staining of the nitrocellulose membrane transferred from SDS-PAGE. Lanes are: NFL input—20% of total amount of NFL used in the pulldown assay; GST: GST incubated with NFL; GST-SacsJ: GST-SacsJ incubated with NFL. Bands stained by Ponceau red in each lane are NFL, GST, GST–SacsJ and NFL (small arrow). (B) Immunolabelling of the nitrocellulose membrane shown in (A) using anti-NFL antibody (NR4, 1/1000) demonstrating that the small band identified in (A) using GST-SacsJ as bait is indeed NFL.