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. 2022 Dec 12;23(24):15742. doi: 10.3390/ijms232415742

Figure 4.

Figure 4

SacsJ domain did not act as a chaperone to reduce heat-denaturation of catalase or citrate synthase in an in vitro assay. (A) Absorbance at 340 nm of catalase (2 µM) incubated with or without Hsp22 used as a positive control (40 or 60 µg) heated at 60 °C. (B) Coomassie brilliant blue staining of a SDS-PAGE of purified recombinant SacsJ protein used in these assays. (C) Absorbance at 340 nm, as a function of time in an assay for heat-denaturation of catalase (2 µM) at 60 °C compared to heat-induced denaturation in the presence of increasing concentrations of SacsJ-GST (1–6 µM). (D,E) Absorbance at 340 nm of catalase (2 µM) or at 320 nm citrate synthase (CS) (0.2 µM) incubated with or without GST (1–6 µM) heated at 60 °C for catalase or 45 °C for CS. (F,G) Absorbance at 340 nm and 320 nm of catalase (2 µM) or citrate synthase (CS) (0.2 µM) incubated with or without SacsJ-GST (1–6 µM) heated at 60 °C for catalase or 45 °C for CS. * p < 0.05 vs. catalase or CS alone, one-way ANOVA, HSD Tuckey post hoc analysis (n = 3).