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. 2022 Dec 12;23(24):15742. doi: 10.3390/ijms232415742

Figure 5.

Figure 5

The cell-permeant peptide, Sacs-myc-TAT, induced step-wise disruption of the vimentin IF network in MCH74 fibroblasts. (A) Representative 3-dimensional reconstructions of Z-stack confocal images of MCH74 fibroblasts double labelled with anti-myc (rabbit anti-myc) and anti-vimentin (V9 mouse monoclonal) treated with increasing concentrations of Sacs-myc-TAT as indicated (0.5 to 5 µM) or control peptide GST-myc-TAT. Treatment with Sacs-myc-TAT for 30 min resulted in nuclear rings of bundled vimentin IF (large arrow). Scale bar: 20 µm. (B) Quantitation of the percentage of fibroblasts presenting circum-nuclear IF bundles or finely distributed IF when treated with increasing concentrations of Sacs-myc-TAT (0.5 to 5 µM) showing an increase in the percentage of cells with IF concentrated surrounding the nucleus. * p < 0.05 vs. NFL alone, one-way ANOVA (n = 3). Note the diffuse distribution of labelling of the myc tag on SacsJ.