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. 2022 Dec 12;23(24):15742. doi: 10.3390/ijms232415742

Figure 8.

Figure 8

The cell-penetrating peptide, SacsJ-myc-TAT, resolved NFL bundles in Sacs−/− motor neuron in culture. (A) Representative 3-dimensional reconstructions of Z-stack confocal images of motor neurons in Sacs−/− 6 week-old spinal cord-DRG cultures double labelled with anti-myc (rabbit anti-myc) and anti-NFL to show the NF network and SacsJ-myc-TAT distribution in motor neurons. Cultures were treated with SacsJ-myc-TAT (0.5 µM) or GST-myc-TAT control peptide for 30 min and compared to untreated cultures. Treatment with SacsJ-myc-TAT resolved the NF bundles (large arrow). Scale bar: 20 µm. (B) Quantitation of the percentage of motor neurons presenting a filamentous or bundled NF network when treated with Sacs-myc-TAT (0.5 µM). * p < 0.05 vs. no treatment or treated with GST-myc-TAT (0.5 µM) using a one-way ANOVA, HSD Tuckey post hoc analysis (n = 3).