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. 2022 Dec 12;23(24):15742. doi: 10.3390/ijms232415742

Figure 9.

Figure 9

SacsJ acts independently to disassemble NF, but requires Hsp70 to degrade NFL. SacsJ-myc or SacsJH33Q-myc, a variant unable to bind HSP70, were expressed in motor neurons in Sacs−/− 6 week-old spinal cord-DRG cultures. (A) Representative 3-dimensional reconstructions of Z-stack confocal images of motor neurons double labelled with anti-myc (rabbit anti-myc) and anti-NFL to show the NF network and expression of NFL. The NF network was dismantled or absent when SacsJ-myc was expressed while SacsJH33Q-myc only dismantled NF network. Scale bar: 10 µm. (B) Quantitation of the percentage of motor neurons presenting a filamentous, bundled, dismantled or no NF network when expressing SacsJ-myc or SacsJH33Q-myc. * p < 0.05 vs. control, ** p < 0.05 vs. SacsJ using a one-way ANOVA, HSD Tuckey post hoc analysis (n = 3).