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. 2000 Dec;68(12):6883–6890. doi: 10.1128/iai.68.12.6883-6890.2000

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Copyright © 2000, American Society for Microbiology

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FIG. 2 — Levels of surface markers on iDC with and without treatment with reagents. iDC were incubated for 2 days in medium containing GM-CSF (500 IU/ml) and the following materials: GM-CSF alone (none) (▨), emulsion buffer (15 μl/ml) (□), TNF-α (100 IU/ml) (▩), IL-1β (100 ng/ml) (), BCG-CWS (15 μg/ml) (■), heat-killed BCG (iDC/bacterium ratio = 1:1) ( ), or viable BCG (iDC/bacterium ratio = 1:1) (▩). Values are expressed as the mean fluorescence intensities measured by a flow cytometer, and the mean values of subclass control MAbs were negligible (∼3 to 4). This experiment was repeated three times with similar results, and representative results are shown.

Inline graphic — Levels of surface markers on iDC with and without treatment with reagents. iDC were incubated for 2 days in medium containing GM-CSF (500 IU/ml) and the following materials: GM-CSF alone (none) (▨), emulsion buffer (15 μl/ml) (□), TNF-α (100 IU/ml) (▩), IL-1β (100 ng/ml) (), BCG-CWS (15 μg/ml) (■), heat-killed BCG (iDC/bacterium ratio = 1:1) ( ), or viable BCG (iDC/bacterium ratio = 1:1) (▩). Values are expressed as the mean fluorescence intensities measured by a flow cytometer, and the mean values of subclass control MAbs were negligible (∼3 to 4). This experiment was repeated three times with similar results, and representative results are shown.