FIG. 3.

Cytokine production by iDC and DC treated with BCG-CWS. Monocytes were cultured in medium containing GM-CSF and IL-4, and the medium was collected every 3 days (0-3, 3-6, 6-9). iDC cultured for 3, 6, or 9 days were incubated in medium with GM-CSF (500 IU/ml) and BCG-CWS (15 μg/ml), and each medium was collected after 2 days (3-5, 6-8, 9-11). The concentrations of each cytokine in the medium (IL-1β [□], TNF-α [■], IL-6 [▨], and IL-12 p40 [▩]) were determined by ELISA. Values are the means ± standard deviations (SD) of triplicate determinations. The concentrations of IL-12 p40 are on the right vertical axis.