FIG. 7.

Signaling of BCG-CWS via TLR. Thioglycolate-elicited peritoneal macrophages from wild-type (1; ■), TLR2-deficient (2; □), TLR4-deficient (3; ▩), or TLR2- and TLR4-deficient (4) mice were cultured with IFN-γ (30 U/ml) in the presence or absence of BCG-CWS (10 μg/ml) for 24 h. Concentrations of TNF-α in the culture supernatants were measured by ELISA. ND, not detected. This experiment was repeated twice with similar results, and representative results are shown.