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. 2022 Dec 9;23(24):15602. doi: 10.3390/ijms232415602

Figure 1.

Figure 1

Comparison of temperature-controlled repeated thermal stimulation (TRTS)-induced neurite-bearing cells (%) among PC12 parental, PC12-P1F1, and PC12-P1D10 cell lines. (a) We evaluated temperature changes in the culture medium during TRTS. Briefly, 24 h before thermal evaluation, a cell-free culture medium was transferred into a 24-well culture plate. Then, temperatures of the culture medium during TRTS (18 h/day) were recorded every 60 s for 24 h. The data represent the average temperature changes of four independent replicates. (b) Cells were exposed to TRTS for 18 h per day for 7 days, and the percentage of neuritogenesis was evaluated. Representative phase-contrast micrographs of cultured cells and the data of neurite-bearing cells on day 7 with or without TRTS of three cell lines: PC12 parental cells (PC12-PA), PC12-P1F1 cells, and PC12-P1D10 cells. Scale bars: 100 μm. The data represent the means ± standard deviation of three replicates. * p < 0.05 vs. control; ** p < 0.01 vs. control; n.s., not significant vs. control.