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. 2022 Dec 11;23(24):15707. doi: 10.3390/ijms232415707

Figure 5.

Figure 5

MSTN regulated the TCA cycle via Smad2/3. (a) Analysis of Smad2/3 binding sites in Idh2, Idh3a, and Idh3b promotor sequences. The red box line is the binding site selected in this study. (b) Expression of Idh2 and Idh3a mRNA in the quadriceps of WT and Mstn−/− mice. (c) The proteins of 6 WT and Mstn−/− mice were collected and triplicated in three lanes to show the protein expression of Smad2/3 and MSTN. (d) The protein expression of Smad2/3 and MSTN in (c) was quantitatively analyzed. Each dot presents a repeat. (e) ChIP-qPCR detected the binding of Smad2/3 to the Idh2 and Idh3a promoter regions in Mstn−/− mice quadriceps. (b,d,e) The data are presented as mean ± SD. Compared with the control group, * p < 0.05, ** p < 0.01, *** p < 0.001; Student’s t-tests were used to calculate the p-values. We used n = 6 mice per group. Except where noted, each dot represents a mouse.