Table 1.
Comparison of V(D)J clonal rearrangements at IGH locus identified by scRNA-seq and amplicon-based NGS from bulk DNA in the 17/19 samples in which the two methods gave matching results.
| Patient ID | scRNA-Seq | Amplicon-Based NGS from Bulk DNA | Common CDR3 | ||||
|---|---|---|---|---|---|---|---|
| IGHV | IGHJ | Proportion | IGHV | IGHJ | Proportion | ||
| PLC-01 | IGHV3-48 | IGHJ4 | 92.90% | IGHV3-30*09 | IGHJ4*02 | 67.55% | CARDSYEDYVYW |
| PLC-02 | IGHV3-21 | IGHJ4 | 97.93% | IGHV3-21*01 | IGHJ4*02 | 74.39% | CARYQLDAVAGKWGHYFDYW |
| PLC-03 | IGHV3-43 | IGHJ5 | 97.77% | IGHV3-9*01 | IGHJ4*02 | 66.23% | CAKARLPLVGGLDSW |
| PLC-05 | IGHV3-43 | IGHJ6 | 49.79% | IGHV3-9*01 | IGHJ6*02 | 13.62% | CTRVIGSGASCYDCYYHGMDVW |
| PLC-06 | IGHV3-53 | IGHJ5 | 87.91% | IGHV3-53*01 | IGHJ4*02 | 57.58% | CARGLTAPGFPLDSW |
| PLC-07 | IGHV3-23 | IGHJ6 | 65.26% | IGHV3-23*01 | IGHJ6*01 | 56.92% | CAKGRADCTDGVCYRRYGMDVW |
| PLC-08 | IGHV3-43 | IGHJ4 | 99.44% | IGHV3-43*01 | IGHJ4*02 | 81.44% | CVKGQGGYTYGGFDCW |
| PLC-10 | IGHV5-51 | IGHJ4 | 77.63% | IGHV5-51*01 | IGHJ4*02 | 57.39% | CARTNWPYYFDHW |
| PLC-12 | IGHV3-43 | IGHJ4 | 93.73% | IGHV3-9*01 | IGHJ4*02 | 62.86% | CARDRYQLIIYYFDRW |
| PLC-15 | IGHV3-43 | IGHJ4 | 98.97% | IGHV3-9*01 | IGHJ4*02 | 32.77% | CAKDVRYGYGSTQSAGFDYW |
| PLC-17 | IGHV4-39 | IGHJ4 | 95.53% | IGHV4-39*07 | IGHJ4*02 | 22.10% | CARDKTTMTFSSPIFDYW |
| PLC-18 | IGHV2-5 | IGHJ1 | 95.78% | IGHV2-5*02 | IGHJ1*01 | 56.58% | CAHSGSMWSGYAGTEYFQHW |
| PLC-19 | IGHV4-59 | IGHJ4 | 67.50% | IGHV4-59*01 | IGHJ4*02 | 21.08% | CARAGDYDLLLLDYW |
| PLC-21 | IGHV5-51 | IGHJ6 | 80.02% | IGHV5-51*03 | IGHJ6*03 | 56.06% | CARLPQGGYYYMDVW |
| PLC-22 | IGHV3-53 | IGHJ5 | 85.10% | IGHV3-53*01 | IGHJ4*02 | 77.01% | CARGLTAPGFPLDSW |
| PLC-23 | IGHV3-33 | IGHJ1 | 56.11% | IGHV3-30-3*02 | IGHJ1*01 | 61.13% | CAFAIGADGEYFQHW |
| PLC-24 | IGHV2-70 | IGHJ4 | 69.68% | IGHV2-70*01 | IGHJ4*02 | 40.67% | CARGASETQVAMSTAELYFFDSW |