Figure 3.
Long-lasting consequences of a single-dose treatment with ONC201 on the mitochondrial nucleoids, mtDNA, and mitochondrial morphology. (A), Average number of mitochondrial nucleoids per cell before, following single-dose 10 µM ONC201 treatment (+ONC) and subsequent washout (washout); (B), Ratio of mtDNA gene MT-ND1 to nDNA encoded gene ACTB normalized to control (qPCR data); (C), Average size of mitochondria in 10 µM ONC201 treated cells. Shown are the mean +/− SD obtained from at least three independent experiments. Statistics conducted using one-way ANOVA, post-hoc Bonferroni test, * p < 0.05, “ns”, non-significant.