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. 2022 Dec 8;23(24):15551. doi: 10.3390/ijms232415551

Figure 4.

Figure 4

Time-dependent effect of single dose ONC201 exposure of BT474 cells on proliferation and expression of markers of ISR. (A), Proliferation of BT474 exposed to 10 µM ONC201 and following washout (marked with arrows). (B), Representative Western blots of markers of ISR (ATF4 and CHOP) and stress-protein GDF-15 following treatment with 10 µM of ONC201 (+ONC201) and subsequent 120 h washout (−ONC201(washout)); (C), Representative Western blots of mitochondrial proteins following treatment with 10 µM of ONC201 (+ONC201) and subsequent 120 h washout (−ONC201(washout)). Shown are mean ± SD (A) and representative Western blots (B,C) from at least three independent experiments.