Figure 3.

Bispecific MerTK/EGFR antibodies and their binding capabilities. (a) Simultaneous binding of hMerTK and EGFR via BLI. Antibodies were loaded onto AHC biosensors and then associated first to MerTK and then to EGFR for 300 s. The colour-coding is the same as represented in the legend for (d). Cell titration on (b,c) A431 and (d) HEK293 cell lines by staining the antibodies with anti-human Fc PE and plotting the relative fluorescence units (RFU) versus the antibody concentration. The negative controls incubated only with secondary antibodies are shown as “NC”. Error bars represent biological duplicates.