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. 2022 Dec 10;23(24):15673. doi: 10.3390/ijms232415673

Figure 4.

Figure 4

EGF competition and inhibition through 7D9G. EGF competition assay via BLI for (a) KiH MerK28-7D9G and (b) MerK28-7D9G. Antibodies were loaded onto AHC biosensors and either associated to 300 nM EGFR (dark red or orange), 300 nM EGF pre-incubated with 1 µM EGF (lighter red and orange), or only PBS (grey). (c) HTRF pAKT of A549 cells stimulated with 20 ng/mL EGF after pre-incubation with the indicated antibodies and respective concentrations. Negative control (NC) represents unstimulated cells, while EGF control shows maximum activation of pAKT. (d) Investigation of MerTK signalling pathway on HEK293 cells after 30 min incubation with either parental MerK28 (blue) or KiH MerK28-7D9G (red). Error bars represent the standard deviation of biological duplicates.