KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| EDC4 | Cell Signaling Technologies | cat#2548S |
| G3BP1 | Santa Cruz Biotechnology | cat#sc-365338 |
| EDC3 | Cell Signaling Technologies | cat#14495 |
| FLAG-tag | Cell Signaling Technologies, Sigma Aldrich | cat#8146, cat#SAB4301135 |
| HA-tag | Invitrogen | cat#26183 |
| GFP | GenScript | cat#A01388 |
| GAPDH | Cell Signaling Technologies | cat#2118 |
| Alexa Fluor® 488 Conjugate Anti-Rabbit IgG (H+L) F(ab’)2 Fragment | Cell Signaling Technologies | cat#4412S |
| IgG (H+L) Goat anti-Mouse, DyLight™ 550 | Invitrogen | cat#84540 |
| Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight™ 650 | Invitrogen | cat#84545 |
| Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight™ 550 | Invitrogen | cat#84541 |
| Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight™ 650 | Invitrogen | cat#84546 |
| Bacterial and virus strains | ||
| XL1-Blue | Agilent | cat#200228 |
| Chemicals, peptides, and recombinant proteins | ||
| Polyethylenimine | Polysciences | cat#24765-1 |
| PBS | Cytiva | cat#SH30378.03 |
| Sodium (meta)arsenite | Sigma-Aldrich | cat#S7400 |
| Hydrogen peroxide | Macron | cat#MK5240120 |
| HHS-465 | Hahm, et al.26 | N/A |
| DAPI | Tocris | cat#5748 |
| Lipofectamine 2000 | Thermo Fisher Scientific | cat#11668019 |
| QuickExtract DNA Extraction Solution | Lucigen Corporation | cat#QE09050 |
| Protease and Phosphatase Inhibitor Mini Tablets | Pierce | cat#A32959 |
| μ Columns | Miltenyi Biotec | cat#130-042-701 |
| Trypsin/Lys-C (LC-MS grade) | Promega | cat#V5073 |
| Dithiothreitol | Fisher bioreagents | cat#BP172-5 |
| Iodoacetamide | Thermo Fisher Scientific | cat#AC122270250 |
| Formaldehyde solution | Sigma-Aldrich | cat#252549 |
| Triton™ X-100 | Sigma-Aldrich | cat#X100 |
| Tween™ 20 | Thermo Fisher Scientific | cat#bp337500 |
| VECTASHIELD® HardSet™ Antifade Mounting Medium | Vector Laboratories | cat#H-1400-10 |
| VECTASHIELD Vibrance® Antifade Mounting Medium | Vector Laboratories | cat#H-1700 |
| Emetine dihydrochloride | Abcam | cat#ab141478 |
| Desthiobiotin-PEG-3-azide | Hahm, et al.26 | N/A |
| Critical commercial assays | ||
| μMACS™ DYKDDDDK Isolation Kit | Miltenyi Biotec | cat#130-101-591 |
| High-Select™ TiO2 Phosphopeptide Enrichment Kit | Thermo Scientific | cat#A32993 |
| QIAprep Spin Miniprep Kit | Qiagen | cat#27106X4 |
| Experimental models: Cell lines | ||
| HEK293T | ATCC | cat#CRL-3216 |
| HeLa | Gift from Dr. Ralph Kleiner (Princeton University) | N/A |
| HEK293T EDC3-KO | This paper | N/A |
| Oligonucleotides | ||
| CACCGTCAGGGAAGAGTGTCAGCTG (Oligo 1 fWd) | Integrated DNA Technologies | N/A |
| AAACCAGCTGACACTCTTCCCTGAC (Oligo 1 rev) | Integrated DNA Technologies | N/A |
| CACCGAACATGGCTACAGATTGGCT (Oligo 2 fWd) | Integrated DNA Technologies | N/A |
| AAACAGCCAATCTGTAGCCATGTTC (Oligo 2 rev) | Integrated DNA Technologies | N/A |
| ACACTTGGTACAGAGCCTGAT (Surveyor oligo 1 fWd) | Integrated DNA Technologies | N/A |
| AAGGGGAGGCCAAGGAATGAA (Surveyor oligo 1 rev) | Integrated DNA Technologies | N/A |
| Recombinant DNA | ||
| EDC3 in pcDNA3.1+/C-(K)DYK | Hahm, et al.26 | N/A |
| EDC3 Y475F in pcDNA3.1+/C-(K)DYK | Hahm, et al.26 | N/A |
| pcDNA3-HA-14-3-3 beta | pcDNA3-HA-14-3-3 beta was a gift from Michael Yaffe (Addgene plasmid # 13270; http://n2t.net/addgene:13270; RRID:Addgene 13270) | cat#13270 |
| HA-14-3-3 zeta | HA-14-3-3 zeta was a gift from FENG-QIAN Li & Ken-Ichi Takemaru (Addgene plasmid # 116888; http://n2t.net/addgene:116888; RRID:Addgene 116888) | cat#116888 |
| HA-14-3-3 epsilon | HA-14-3-3 epsilon was a gift from FENG-QIAN Li & Ken-Ichi Takemaru (Addgene plasmid # 116886; http://n2t.net/addgene:116886; RRID:Addgene 116886) | cat#116886 |
| HA-14-3-3 eta | HA-14-3-3 eta was a gift from FENG-QIAN Li & Ken-Ichi Takemaru (Addgene plasmid # 116887; http://n2t.net/addgene:116887; RRID:Addgene 116887) | cat#116887 |
| BFP-Dcp1a | BFP-Dcp1a was a gift from Gia Voeltz (Addgene plasmid # 153973; http://n2t.net/addgene:153973; RRID:Addgene 153973) | cat#153973 |
| GFP-Dcp1b | GFP-Dcp1b was a gift from Gia Voeltz (Addgene plasmid # 153976; http://n2t.net/addgene:153976; RRID:Addgene 153976) | cat#153976 |
| Software and algorithms | ||
| Custom LC-MS Bioinformatics Pipeline | Hahm, et al.26 | N/A |
| Skyline | MacCoss Lab Software | https://skyline.ms/project/home/software/Skyline/begin.view |
| Byonic | Protein Metrics | https://proteinmetrics.com/byos/ |
| Fiji | ImageJ | https://github.com/fiji/fiji |
| GraphPad PRISM | GraphPad | https://www.graphpad.com/ |
| RawConverter | RawConverter | http://fields.scripps.edu/rawconv/ |
| Adobe Illustrator | Adobe | https://www.adobe.com/products/illustrator.html |
| Microsoft Office | Microsoft | https://www.office.com/ |
| Image Lab | Bio-rad | https://www.bio-rad.com/en-us/product/image-lab-software?ID=KRE6P5E8Z |
| MestreNova | Mestrelab Research | https://mestrelab.com/download/mnova/ |
| Leica Applications Suite | Leica Microsystems | https://www.leica-microsystems.com/products/microscope-software/p/leica-application-suite/ |
| Deposited data | ||
| 10.6019/PXD038010 | This paper | PXD038010 |