Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| cell line (Homo-sapiens) | HEK293T | ATCC | Cat#: CRL-3216 | |
| cell line PACC1 knockout (Homo-sapiens) |
PAC KO HEK293T | doi:10.1126/science.aav9739 | ||
| recombinant DNA reagent | pIRES2-EGFP-hPAC | doi:10.1126/science.aav9739 | ||
| commercial assay or kit | Lipofectamine2000 | Invitrogen | Cat#: 11668019 | |
| commercial assay or kit | QuikChange II XL site-directed mutagenesis |
Agilent Technologies | Cat#: 200522 | |
| software, algorithm | pCLAMP 10.7 | Molecular Devices | RRID:SCR_011323 | |
| software, algorithm | Clampfit 10.7 | Molecular Devices | ||
| software, algorithm | PyMOL 2.3.4 | Schrodinger, LLC | ||
| software, algorithm | Gromacs 2020.1 | doi: 10.1016/j.softx.2015.06.001 | ||
| software, algorithm | CHARMM-GUI | doi: 10.1002/jcc.23702 | ||
| software, algorithm | PPM Web Server | doi: 10.1093/nar/gkr703 | ||
| software, algorithm | Python | Python Software Foundation |
||
| software, algorithm | GraphPad Prism 8 | GraphPad |