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Restriction map and construction of plasmids containing the mgl gene and its adjacent region. pME220 contains the entire mgl gene and its upstream region. pME221 contains the flanking region of the mgl gene. pMF440 contains the 6.2-kb ClaI-BamHI fragment reconstructed from pME220 and pME221. pME441 contains the mgl gene interrupted by an ermF-ermAM cassette. pME441 was linearized with NotI and XhoI and introduced into P. gingivalis W83 by electroporation.
