Figure 4.

The target relationship verification of miR-200c and PANK3 using dual luciferase reporter assay. (A) Homology analysis of miR-200c in different species (B) The structural diagram of wild-type (WT) and mutant-type (MUT) dual luciferase reporter vectors. (C) The figure on the left indicates the results from agarose gel electrophoresis when WT and MUT pmirR-RB-Report^TM vectors were digested with the restriction endonucleases NotI and Xhol. The figure on the right shows the results from Sanger sequencing. The miR-200c was indicated with a pink irregular shape above sequencing figure. (D) The renilla/firefly activity was measured when miR-200c mimic or miR-200c NC, and wild-type or mutated pmirR-RB-Report^TM vectors were co-transfected into HEK293T cells. The values represent mean ± SD (n = 3), * p < 0.05.