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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2022 Nov 3;43(1):79–91. doi: 10.1161/ATVBAHA.121.317735

Figure 2: Platelets from aged pSOD2-KO mice exhibit early and sustained increases in calcium.

Figure 2:

Washed platelets from young or aged mice deficient in platelet SOD2 (pSOD2-KO) or littermate control mice were loaded with Fluo-4 dye, resuspended in m-Tyrode buffer containing 1 mM calcium chloride and analyzed on BD Accuri C6 flow cytometer at baseline (0 to 45 sec) and upon activation with thrombin (45 to 240 sec) in real time. A. Cumulative tracings of calcium levels (0–240 sec). B. Quantification of cumulative fluorescent signals at baseline (0–45 sec) and during thrombin activation (from 45–240 sec). C. Quantification of cumulative fluorescent signals (45 sec to 120 sec). N = Cumulative data from 5–7 mouse in each group. Data are presented as Mean ± SE and analyzed for cumulative signal using two-way ANOVA with Tukey’s multiple comparison test.