FIG. 3.

Internalization of live and dead E. cuniculi spores by MRC5 cells. Two million either live or formaldehyde-fixed spores were added to each well of confluent cells and coincubated for 2 h. Intracellular spores were identified using differential immunofluorescence staining. The rate of uptake of spores differed markedly between experiments. However, there was no statistically significant difference in the rate of uptake between live and formaldehyde-fixed spores (P ≫ 0.05 by Student's t test). Exp, experiment.