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. 2022 Dec 22;7:75. doi: 10.1038/s41536-022-00269-w

Fig. 3. Characterization of the dECM hydrogel.

Fig. 3

a Gross appearance of the articular cartilage derived from the pig knee joint. b Representative images of the dECM hydrogels. c–f Various concentrations (10, 20, 30, 40 mg/mL, respectively) of the dECM hydrogels at 4 °C. g–j Various concentrations of the dECM hydrogels (10, 20, 30, 40 mg/mL, respectively) at 37 °C. k, l Representative images of articular cartilage characterization pre- and post-decellularization by DAPI staining. m, n Representative images of articular cartilage characterization pre- and post-decellularization by H&E staining. Scale bar = 100 μm. h Representative images of the dECM hydrogels (20×). Scale bar = 100 μm. o SEM images of various concentrations (10, 20, 30, and 40 mg/mL) of the dECM hydrogels. q DNA content in the ECM and dECM, which confirmed successful decellularization. r FTIR spectroscopy of the decellularized ECM (d-ECM) and dECM hydrogels. s, t GAG and Collagen II (COL-II) concentration of the ECM and dECM. u Rheological behavior of the dECM hydrogels. ns, not significant; *P < 0.05, **P < 0.01, ***P < 0.001, * is the statistical difference compare with ECM group. One-way ANOVA followed by the Tukey post hoc test was used. Each data point represents average ± standard deviation, n = 4.