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. 2022 Dec 22;5:1402. doi: 10.1038/s42003-022-04349-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Negative stained-EM of SF-sEVs and PC3 sEVs. b Western blot results for sEV markers TSG-101, CD63, CD81, and CD9. The ER marker calnexin was targeted as a negative control. c NTA analysis of the mode particle diameter recorded values of 155.8 and 192.2 nm for SF-sEVs and PC3 sEVs, respectively. d The quality of the purification was evaluated by gel electrophoresis for PC3 sEVs and SF-sEVs Repl 2 and Repl 3. A: Total minus surface fraction PC3 sEVs; B: Total minus surface fraction SF-sEVs Repl 2; C: Total minus surface SF-sEVs fraction Repl 3; D: Surface SF-sEVs fraction Repl 2; E: Surface SF-sEVs fraction Repl 3; F: Surface fraction PC3 sEVs.