Table 1.
Cellular markers used in HCS panels.
| Cellular target | Dye/marker | Excitation maxa | Emission maxa | Supplier/Cat. number |
|---|---|---|---|---|
| Panel A—cell line: Parental U-2 OS cells | ||||
| Nucleus | Hoechst 33342 | 350 | 453 | Thermo Fisher Scientific/62249 |
| RNA and nucleoli | SYTO14 Green | 505 | 524 | Thermo Fisher Scientific/S7576 |
| Plasma membranes and Golgi | WGA Alexa Fluor® 555 Conjugate | 555 | 568 | Thermo Fisher Scientific/W32464 |
| Mitochondria | MitoTracker® DeepRed FM | 641 | 662 | Thermo Fisher Scientific/M22426 |
| Panel B—cell line: U2OS-mOrange2-Peroxisome cells | ||||
| Nucleus | Hoechst 33342 | 350 | 453 | Thermo Fisher Scientific/62249 |
| Lysosomes | LysoTracker Green DND-26 | 500 | 510 | Thermo Fisher Scientific/L7526 |
| Peroxisomes | mOrange2 (genetically encoded) | 548 | 562 | Addgene/54596 |
| Lipid droplet | LipidTOX™ HCS LipidTOX DeepRed | 634 | 652 | Thermo Fisher Scientific/H34477 |
| Panel C1—cell line: U2OS-LMNB1-TUBA1B-ACTB cells | ||||
| Nucleus | DRAQ5 | 596 | 696 | Thermo Fisher Scientific/62251 |
| ER | ER-Tracker™ Blue-White DPX | 371 | 557 | Thermo Fisher Scientific/E12353 |
| Panel C2—cell line: U2OS-LMNB1-TUBA1B-ACTB cells | ||||
| Nucleus | Hoechst 33342 | 350 | 453 | Thermo Fisher Scientific/62249 |
| Actin | Alexa Fluor™ 568 Phalloidin | 578 | 603 | Thermo Fisher Scientific/A12380 |
| Tubulin | TUBA1B-GFP (genetically encoded) | 489 | 509 | NA |
aAccording to Fluorescence Spectra Viewer (Thermo Fisher Scientific).