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. 2022 Dec 22;12:22140. doi: 10.1038/s41598-022-26690-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Panel (a): Absorption spectra of NTBD in DMSO: H₂O solutions with various – 1:99, 1:9, 4:6, 5:5, 6:4, 9:1 and 99:1 – volume ratio. Panel (b): Correlation between the ratio of absorbance at the wavelength of 319 nm to the one at 331 nm and the ratio of DMSO to H₂O in mixed solution. Panel (c): Fluorescence emission spectra of NTBD in DMSO: H₂O solutions with various – 1:99, 1:9, 2:8, 4:6, 5:5, 6:4, 7:3 and 8:2 – volume ratio. The excitation was set at the absorbance maximum of each sample, respectively as pointed in the figure. Panel (d): Correlation between the ratio of fluorescence emission at the wavelength of 379 nm to the one at 444 nm along with the ones at 444 nm to 502 nm and the ratio of DMSO to H₂O in mixed solution. The experiments were conducted at room temperature.