Table 2.
Fluorescence lifetimes τ [ns], fractional intensities f and standard deviation measured for NTBD at various DMSO: H2O volume ratio.
| DMSO: H2O Em (Ex 315) filter 320 |
τ1 ± sd [ns] | f1 ± sd | τ2 ± sd [ns] | f2 ± sd |
|---|---|---|---|---|
| 99:1 | 1.203 ± 0.033 | 1.000 ± 0.000 | ||
| 9:1 | 0.873 ± 0.005 | 1.000 ± 0.000 | ||
| 7:3 | 0.435 ± 0.035 | 0.756 ± 0.075 | 1.867 ± 0.588 | 0.245 ± 0.075 |
| 5:5 | 0.355 ± 0.009 | 0.432 ± 0.013 | 1.673 ± 0.050 | 0.568 ± 0.013 |
| 3:7 | 0.213 ± 0.055 | 0.342 ± 0.044 | 1.253 ± 0.064 | 0.658 ± 0.044 |
| 1:9 | 0.129 ± 0.102 | 0.583 ± 0.206 | 0.686 ± 0.250 | 0.612 ± 0.366 |
| 1:99 | 0.158 ± 0.063 | 0.571 ± 0.067 | 3.240 ± 0.287 | 0.370 ± 0.091 |
| DMSO: H2O Em (Ex 331) filter 450 |
τ1 ± sd [ns] | f1 ± sd | τ2 ± sd [ns] | f2 ± sd |
|---|---|---|---|---|
| 99:1 | 1.042 ± 0.159 | 1.000 ± 0.000 | ||
| 9:1 | 0.115 ± 0.106 | 1.000 ± 0.000 | ||
| 7:3 | 0.760 ± 0.239 | 0.372 ± 0.128 | 1.960 ± 0.221 | 0.612 ± 0.140 |
| 5:5 | 1.445 ± 0.035 | 1.000 ± 0.000 | ||
| 3:7 | 1.146 ± 0.021 | 1.000 ± 0.000 | ||
| 1:9 | 0.651 ± 0.013 | 1.000 ± 0.000 | ||
| 1:99 | 0.441 ± 0.193 | 0.402 ± 0.076 | 2.721 ± 0.561 | 0.732 ± 0.216 |