Table 3.
Echocardiographic data for Lmnap.H222P/H222P (H222P) mice treated with tubastatin A or DMSO at 2, 2.5, and 3 months of age
| H222P | H222P | H222P | H222P | H222P | H222P | |
|---|---|---|---|---|---|---|
| DMSO | Tubastatin | DMSO | Tubastatin | DMSO | Tubastatin | |
| Age | 2 months | 2 months | 2.5 months | 2.5 months | 3 months | 3 months |
| n | 5 | 6 | 5 | 6 | 5 | 6 |
| Time (ms) | 99.83 ± 1.65 | 100.4 ± 1.75 | 101.0 ± 1.56 | 100.4 ± 1.72 | 102.3 ± 2.09 | 100.7 ± 1.35 |
| Heart rate (bpm) | 601.7 ± 9.96 | 598.3 ± 10.14 | 594.5 ± 9.31 | 598.7 ± 10.25 | 587.4 ± 11.93 | 596.4 ± 7.94 |
| Cardiac output (l/min) | 0.06 ± 0.007 | 0.07 ± 0.002 | 0.07 ± 0.002 | 0.06 ± 0.003 | 0.06 ± 0.005 | 0.06 ± 0.003 |
| IVSd (cm) | 0.07 ± 0.002 | 0.07 ± 0.02 | 0.07 ± 0.001 | 0.07 ± 0.001 | 0.07 ± 0.002 | 0.07 ± 0.001 |
| LVDd (cm) | 0.37 ± 0.01 | 0.37 ± 0.004 | 0.39 ± 0.002 | 0.37 ± 0.004 | 0.39 ± 0.009 | 0.37 ± 0.003 |
| LVPWd (cm) | 0.07 ± 0.003 | 0.07 ± 0.003 | 0.06 ± 0.003 | 0.07 ± 0.003 | 0.07 ± 0.004 | 0.06 ± 0.002 |
| IVSs (cm) | 0.12 ± 0.004 | 0.12 ± 0.002 | 0.11 ± 0.002 | 0.11 ± 0.002 | 0.11 ± 0.004 | 0.11 ± 0.003 |
| LVDs (cm) | 0.21 ± 0.006 | 0.21 ± 0.004 | 0.24 ± 0.004 | 0.19 ± 0.004**** | 0.26 ± 0.008 | 0.22 ± 0.005**** |
| LVPWs (cm) | 0.0 ± 0.004 | 0.11 ± 0.003 | 0.09 ± 0.004 | 0.11 ± 0.003 | 0.08 ± 0.002 | 0.10 ± 0.005* |
| LVED vol (ml) | 0.13 ± 0.11 | 0.13 ± 0.004 | 0.15 ± 0.003 | 0.12 ± 0.003 | 0.16 ± 0.01 | 0.12 ± 0.005 |
| LVES vol (ml) | 0.02 ± 0.003 | 0.02 ± 0.002 | 0.04 ± 0.002 | 0.02 ± 0.001** | 0.05 ± 0.005 | 0.03 ± 0.003** |
| EF (%) | 82.9 ± 0.46 | 82.45 ± 0.52 | 75.33 ± 0.39 | 82.90 ± 0.58*** | 70.18 ± 2.01 | 78.81 ± 1.11**** |
| FS (%) | 45.65 ± 1.06 | 45.25 ± 0.55 | 38.43 ± 0.33 | 45.68 ± 0.63**** | 34.42 ± 1.51 | 41.60 ± 1.03**** |
| LV eject vol (ml) | 0.10 ± 0.009 | 0.11 ± 0.002 | 0.11 ± 0.002 | 0.10 ± 0,003 | 0.11 ± 0.007 | 0.10 ± 0.003 |
| h/r | 0.37 ± 0.006 | 0.35 ± 0.008 | 0.34 ± 0.007 | 0.38 ± 0.005* | 0.35 ± 0.008 | 0.36 ± 0.004 |
Statistics: one-way ANOVA followed by Tukey’s multiple comparison test. Values are means ± SEM.
IVS inter ventricular septum, LVD left ventricular diameter, LVPW left ventricular posterior wall, LVED left ventricular end diastolic, LVES left ventricular end systolic, EF ejection fraction, FS fractional shortening, s systole, d diastole.
*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and ****p ≤ 0.0001 between age-matched tubastatin A and DMSO treated mice.