Table 2.

Interaction of neuroprotective interventions on antioxidant levels of CIS-depressed brain regions of mice

Treatment group (mg/kg)	Different brain area	LPO (n mole of MDA/mg protein)	Nitrate (μ mole of Nitrite/mg protein)	Catalase (μ mole of H2O2/min/mg protein)	GSH (μ mole of GSH/mg protein)
N A I V E	Hippocampus	0.162 ± 0.023	315 ± 61	0.701 ± 0.034	0.062 ± 0.0017
	Striatum	0.159 ± 0.041	310 ± 12	0.697 ± 0.051	0.058 ± 0.0021
	Cortex	0.165 ± 0.022	319 ± 17	0.708 ± 0.041	0.068 ± 0.0034
CIS	Hippocampus	0.622 ± 0.03a	638 ± 13a	0.123 ± 0.021a	0.014 ± 0.0012
	Striatum	0.618 ± 0.045	633 ± 21a	0.119 ± 0.031a	0.010 ± 0.0037a
	Cortex	0.627 ± 0.062a	642 ± 14a	0.127 ± 0.052 a	0.017 ± 0.0036a
Imip-10 + CIS	Hippocampus	0.186 ± 0.061b	357 ± 19b	0.689 ± 0.046b	0.059 ± 0.0087b
	Striatum	0.175 ± 0.021b	359 ± 12b	0.684 ± 0.016b	0.056 ± 0.008b
	Cortex	0.191 ± 0.062b	362 ± 18b	0.697 ± 0.016b	0.064 ± 0.008b
R-20 + CIS	Hippocampus	0.612 ± 0.019 ns	629 ± 12 ns	0.138 ± 0.025 ns	0.016 ± 0.002 ns
	Striatum	0.607 ± 0.046 ns	631 ± 18 ns	0.124 ± 0.025 ns	0.013 ± 0.003 ns
	Cortex	0.615 ± 0.092 ns	634 ± 16 ns	0.135 ± 0.015 ns	0.019 ± 0.001 ns
R-40 + CIS	Hippocampus	0.326 ± 0.071b	456 ± 17b	0.289 ± 0.017b	0.029 ± 0.006b
	Striatum	0.317 ± 0.017b	458 ± 28b	0.251 ± 0.027b	0.024 ± 0.006b
	Cortex	0.324 ± 0.034b	461 ± 15b	0.271 ± 0.012b	0.037 ± 0.0011b
Res-5 + CIS	Hippocampus	0.614 ± 0.021 ns	626 ± 52 ns	0.142 ± 0.033 ns	0.015 ± 0.0014 ns
	Striatum	0.607 ± 0.033 ns	629 ± 32 ns	0.129 ± 0.019 ns	0.014 ± 0.0010 ns
	Cortex	0.615 ± 0.041 ns	631 ± 14 ns	0.135 ± 0.021 ns	0.017 ± 0.0014 ns
Res-10 + CIS	Hippocampus	0.329 ± 0.052b	458 ± 25b	0.291 ± 0.042b	0.028 ± 0.0028b
	Striatum	0.321 ± 0.043b	461 ± 18b	0.257 ± 0.031b	0.026 ± 0.0037b
	Cortex	0.328 ± 0.034b	463 ± 19b	0.271 ± 0.031b	0.038 ± 0.0028b
17β-E2-40 + CIS	Hippocampus	0.616 ± 0.062 ns	631 ± 32 ns	0.135 ± 0.013 ns	0.017 ± 0.0014 ns
	Striatum	0.611 ± 0.066 ns	633 ± 42 ns	0.121 ± 0.019 ns	0.012 ± 0.0010 ns
	Cortex	0.619 ± 0.081 ns	635 ± 17 ns	0.135 ± 0.022 ns	0.02 ± 0.0014 ns
17β-E2-80 + CIS	Hippocampus	0.330 ± 0.067b	460 ± 25b	0.284 ± 0.032b	0.03 ± 0.0028b
	Striatum	0.320 ± 0.074b	462 ± 26b	0.248 ± 0.021b	0.025 ± 0.0037b
	Cortex	0.327 ± 0.063b	464 ± 15b	0.271 ± 0.011b	0.039 ± 0.0028b
R-40 + Res-10 + CIS	Hippocampus	0.185 ± 0.031b,f,g	369 ± 13b,f,g	0.658 ± 0.15b,f,g	0.057 ± 0.004b,f,g
	Striatum	0.199 ± 0.041b,f,g	371 ± 18b,f,g	0.654 ± 0.011b,f,g	0.054 ± 0.0041b,f,g
	Cortex	0.194 ± 0.034b,h,g	373 ± 11b,f,g	0.674 ± 0.013b,f,g	0.061 ± 0.005b,f,g
R-40 + 17β-E2-80 + CIS	Hippocampus	0.192 ± 0.073b,h,g	374 ± 26b,h,g	0.664 ± 0.024b,h,g	0.056 ± 0.0074b,h,g
	Striatum	0.204 ± 0.057b,h,g	376 ± 17b,h,g	0.651 ± 0.015b,h,g	0.051 ± 0.0093b,h,g
	Cortex	0.197 ± 0.061b,f,g	378 ± 15b,h,g	0.681 ± 0.051b,h,g	0.064 ± 0.0074b,h,g
Res-10 + 17β-E2-80 + IS	Hippocampus	0.196 ± 0.014b,f,g	364 ± 12b,f,g	0.641 ± 0.013b,f,g	0.057 ± 0.0094b,f,g
	Striatum	0.206 ± 0.043b,f,g	366 ± 37b,f,g	0.661 ± 0.019b,f,g	0.053 ± 0.0073b,f,g
	Cortex	0.16 ± 0.023b,f,g	368 ± 19b,f,g	0.678 ± 0.017b,f,g	0.062 ± 0.0014b,f,g

Values are expressed as Mean ± S.E.M. using one-way ANOVA along with Tukey’s test

Where CIS (Chronic immobilization stress); Imp-10 (Imipramine-10 mg/kg, i.p.); R-20 (Rutin-20 mg/kg, p.o.); R-40 (Rutin-40 mg/kg, p.o.); Res-5 (Resveratrol- 5 mg/kg, p.o.); Res-10 (Resveratrol-10 mg/kg, p.o.); 17β-E2-40 (17β-estradiol-40 mg/kg, p.o.); 17β-E2-80 (80 mg/kg, p.o.)

^aMeans p < 0.05 as compared to Naive; ^bMeans p < 0.05 as compared to CIS; ^cMeans p < 0.05 as compared to R-20; ^dMeans p < 0.05 as compared to Res-5; ^eMeans p < 0.05 as compared to 17β-E2-40; ^fMeans p < 0.05 as compared to R-40; ^gMeans p < 0.05 as compared to Res-10; ^hMeans p < 0.05 as compared to 17β-E2-80; ^nsMeans non-significant, i.e. p > 0.05