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OP-D-induced p53 and its target gene p21 in HCT116p53+/+ and HCT116p53−/− cells were treated with OP-D (0, 20, or 40 μM) for 24 h. Cell lysates were performed for p53, p21, and β-actin by Western blotting. Data represent means ± SD. ***p < 0.005, ****p < 0.001 versus untreated control.
