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OP-D induced apoptosis through activating p53 in HCT116p53+/+ cells. HCT116p53+/+ cells were transfected with p53 siRNA for 48 h. After transfected, cells were treated with OP-D (40 μM) for 24 h. Cell lysates were performed for PARP, p21, p53, and β-actin by Western blotting. Data represent means ± SD. **p < 0.01, ***p < 0.005, ****p < 0.001.
