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. 2022 Dec 9;13:974468. doi: 10.3389/fphar.2022.974468

FIGURE 5.

FIGURE 5

OP-D activates p53 via RPL5 or RPL11 by causing nucleolar stress in HCT116p53+/+ cells. HCT116p53+/+ cells were transfected with (A) L5 or (B) L11 siRNA for 48 h. After being transfected, cells were treated with OP-D (40 μM) during for 24 h. Cell lysates were performed for p53, p21, L5, L11, and β-actin by Western blotting. Data represent means ± SD. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001. HCT116p53+/+ cells were treated with OP-D (40 μM) for 24 h. Then, cells were fixed with 4% paraformaldehyde and stained with (C) IPO7 or (D) XPO1 antibody. Images are magnification 400 ×, scale bar 50 µm.