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. 2022 Dec 23;13:7907. doi: 10.1038/s41467-022-35590-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a–c The A549 and HEK293T cells were inoculated with rACE2_1-706, followed by SARS-CoV-2 infection. Vehicle-treated cells served as a control. The viral antigens of these cells were detected by immunostaining of Nucleocapsid Protein (NP) (green) as shown in a. (One representative data was shown from three independently repeated experiments) (Scale bar: 20 μm) The viral gene copies of these cells were measured by qPCR analyses of the SARS-CoV-2 genes as shown in b and c. (n = 3 biologically independent experiments) d Experimental strategy to study the role of human solACE in mediating SARS-CoV-2 infection in mice. e Immunostaining of human ACE2 (red) and SARS-CoV-2 (green) Nucleocapsid Protein (NP) in lung sections of mice expressing solACE2_1-706/ solACE2_1-740/ACE2_{full length (FL)} (n = 8 for AAV-control; n = 8 for AAV-hACE2(1-706); n = 8 for AAV-hACE2 (1-740); n = 8 for AAV-hACE2(full length)). (Scale bar: 20 μm) f The viral loads in the lungs of mice expressing solACE2_1-706/ solACE2_1-740/ ACE2_{full length (FL)} were measured by qPCR analyses of the SARS-CoV-2 genes (n = 8 for AAV-control; n = 8 for AAV-hACE2(1-706); n = 8 for AAV-hACE2 (1-740); n = 8 for AAV-hACE2(full length)). g Summary histology scores determined in the lung tissues of mice expressing solACE2_1-706/ solACE2_1-740/ACE2_{full length (FL)} (n = 8 for AAV-control; n = 8 for AAV-hACE2(1-706); n = 8 for AAV-hACE2 (1-740); n = 8 for AAV-hACE2(full length)) h Representative Hematoxylin-eosin (HE) staining of lungs of mice expressing solACE2_1-706/ solACE2_1-740/ACE2_{full length (FL)} (n = 8 for AAV-control; n = 8 for AAV-hACE2(1-706); n = 8 for AAV-hACE2 (1-740); n = 8 for AAV-hACE2(full length)). (Scale bar: 50 μm) Data are means ± S.E.M. of three independent repeats. Statistical analyses were performed by one way ANOVA for (b, c; f, g) Source data are provided as a Source Data file.