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. 2022 Dec 23;12:22191. doi: 10.1038/s41598-022-26473-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of EVs: (A,B) particle size vs. percent intensity graphs of healthy donor (A) and COVID-19 (+) patients (B) SEVs/LEVs. (C) Mean of n = 5 sample size graph is mentioned. (D) TEM images of healthy donor and COVID-19 (+) patients SEVs and LEVs (representative image). An n = 5 each of healthy and COVID-19 SEVs and LEVs was used for particle size and TEM analysis. (E) Western blotting analysis of EV specific tetraspanin markers (CD63 and CD81) of healthy donor SEVs/LEVs and COVID-19 (+) SEVs/LEVs and EDP as negative control.