TABLE 1.

Comparison of several isothermal amplification-based methods developed for SARS-CoV-2 detection.

Name of assay	Principle	Company	Target gene	Sensitivity	Time (min)	References
RT-LAMP assay	RT-LAMP	—	N	100 copies/rxn	30	Baek et al., 2020
mRT-LAMP-LFB	RT-LAMP	—	ORF1ab, N	12 copies/rxn	40	Zhu et al., 2020
COVID-19-LAMP	RT-LAMP	—	ORF3a, E	42 copies/rxn	60	Chow et al., 2020
Suitcase lab	RT-RPA	—	E, N	15 copies/rxn	15	El Wahed et al., 2021
—	RT-RPA	—	N	8 copies/rxn	20	Behrmann et al., 2020
Respiratory Virus Nucleic Acid Detection Kit	RT-LAMP	CapitalBio Technology	ORF1ab, N	150 copies/ml	90	CapitalBio coporation, 2022
Novel Coronavirus (2019-nCoV) Nucleic Acid Detection Kit	CPA	Ustar Biotechnologies	ORF1ab, N	200 copies/ml	80	USTAR, 2022
iAMP COVID-19 Detection Kit	RT-LAMP	Atila BioSystems	ORF1ab, N	4 copies/μl	<90	Rai et al., 2021
ID NOW COVID-19	NEAR	Abbott Diagnostics Scarborough	RdRp	125 copies/ml	15	Abbott Diagnostics, 2020
SHERLOCK CRISPR SARS-CoV-2 Kit	RT-LAMP, CRISPR-Cas13	Sherlock Biosciences	ORF1ab, N	4.5 copies/μl–ORF1ab; 0.9 copies/μl–N	60	Sherlock Biosciences, 2021
SARS-CoV-2 DETECTR Reagent Kit	RT-LAMP, CRISPR-Cas12	Mammoth Biosciences	E, N	10 copies/μl	45	Broughton et al., 2020

CPA, cross priming amplification; NEAR, nicking enzyme amplification reaction.