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Design and results of steepest ascent method.
| Treatment | Beef broth (%) | Peptone (%) | Yeast extract (%) | Glucose (%) | KH₂PO4 (%) | MgSO4 (%) | Protein content (%) |
|---|---|---|---|---|---|---|---|
| 1 | 20 | 0.1 | 0.15 | 1 | 0.2 | 0.02 | 2.09 ± 0.03 |
| 2 | 30 | 0.3 | 0.15 | 2 | 0.2 | 0.02 | 2.17 ± 0.06 |
| 3 | 40 | 0.5 | 0.15 | 3 | 0.2 | 0.02 | 2.06 ± 0.06 |
| 4 | 50 | 0.7 | 0.15 | 4 | 0.2 | 0.02 | 2.02 ± 0.04 |
| 5 | 60 | 0.9 | 0.15 | 5 | 0.2 | 0.02 | 1.75 ± 0.11 |
