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. 2022 Dec 9;13:1055497. doi: 10.3389/fimmu.2022.1055497

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Copyright © 2022 Chu, Talano, Baxter-Lowe, Verbsky, Morris, Mahanti, Ayello, Keever-Taylor, Johnson, Weinberg, Shi, Moore, Fabricatore, Grossman, van de Ven, Shenoy and Cairo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunophenotyping of mononuclear cells added back using flow cytometry. The final products for HSC grafts were characterized by staining with antibodies against: CD3, CD56, CD19, lineage markers (CD3, CD14, CD16, CD19, CD20 and CD56), HLA-DR, CD123, and CD11c. (A) Representative flow cytometry dot plots of T, NK, NKT and B cells (donor 526-015) of WBC. (B) Representative flow cytometry dot plots of CD123⁺ DC and CD11c⁺ DC (donor 526-015) of Lin- (lineage negative) cells. (C) Mean and SEM for the cell subsets of all donors. SEM, standard error of the mean.