Figure 2. Dose-dependent inhibition of TRPV4 channels by salbutamol.
(A) Dose–response for inhibition by salbutamol (5 min) of currents activated by GSK 300 nM (+120 mv). Smooth curve is a fit with the Hill equation (KD = 81.3 µM and Hill coefficient = 1.4). A single salbutamol concentration in the absence of GSK was tested per outside-out membrane patch, and the remaining GSK-activated current was normalized to the current obtained with GSK initially (n = 3–12 for each concentration point). (B) Time course of inhibition by 500 µM salbutamol, τ = 225.6 s (n = 8–10 for each time point). Data were obtained at −60 mV and fit to a single exponential. (C) Recovery from inhibition by salbutamol (500 µM). Current inhibition is irreversible, remaining fraction of currents are shown, and the zero time point represents currents in the presence of GSK101 right after application of salbutamol 500 µM for 5 min.
Source data are available for this figure.
