Figure S3. S688A-TRPV4 channel exhibits time-dependent rundown.

(A) Representative traces of currents at +120 and −120 mV in the presence of GSK 300 nM (black) and GSK 300 nM after salbutamol 500 µM (purple or blue) from TRPV4-S688A channels. (A, B) Representative traces obtained as in (A) but with exposure to only recording solution without salbutamol (blue). (A, B, C) Average data for experiments in (A, B). Data were normalized to the initial value with GSK in WT TRPV4 channels. Current rundown was also assessed for WT channels exposed to recording solution for 5 min, and current inhibition was graphed (black symbols, 60.17% ± 19%; n = 30), TRPV4-S688A (purple symbols) channels exhibited 87.56% ± 10.4% (n = 11) inhibition after salbutamol 500 µM, and TRPV4-S688A (blue symbols) channels exposed to only recording solution exhibited 79.3% ± 19.7% (n = 7) of current inhibition. No statistically significant differences were found between TRPV4-S688A channels exposed to either salbutamol or just recording solutions. Both experimental groups with TRPV4-S688A ion channels exhibited statistical differences versus WT channels. *P = 0.0002 for WT TRPV4 versus TRPV4-S688A with salbutamol and *P = 0.0338 for WT TRPV4 versus TRPV4-S688A without salbutamol, as indicated by brackets. One-way analysis of variance with Tukey’s post hoc test was performed.

Source data are available for this figure.