TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli | ||
| DH5αMCR | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80ΔlacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 deoR thi-1 phoA supE441-gyrA96 relA1 | Gibco-BRL |
| INVαF′ | F′ endA1 recA1 hsdR17(rk−, mk+) supE44 thi-1 gyrA96 relA1 Φ80lacZΔM15 Δ(lacZYA-argF)U169 | Invitrogen |
| N. gonorrhoeae | ||
| FA19 | Wild-type | 40 |
| FA6747 | TbpA− [tbpA::mTn3(cm)] | 16 |
| FA6905 | TbpB− (ΔtbpB) | 18 |
| FA6935 | TbpA TonB-box mutant | 14 |
| FA6815 | TbpA− TbpB− (tbpB::Ω) | 2 |
| MCV201 | ΔL5TbpA | This study |
| MCV202 | ΔL5TbpA TbpB− | This study |
| MCV203 | ΔL4TbpA | This study |
| MCV204 | ΔL4TbpA TbpB− | This study |
| MCV205 | ΔL8TbpA | This study |
| MCV206 | ΔL8TbpA TbpB− | This study |
| Plasmids | ||
| pBSIISK(+) | Ampr | Stratagene |
| pET21 | Ampr | Novagen |
| pCR2.1 | Ampr Kanr | Invitrogen |
| pUNCH412 | pBSIISK(+) containing HindIII to XbaI fragment encoding TbpA | 15 |
| pVCU201 | pCR2.1 containing 5′ half of ΔL5tbpA (HindIII-BamHI) | This study |
| pVCU204 | pCR2.1 containing 3′ half of ΔL5tbpA (BamHI-XhoI) | This study |
| pVCU205 | pET21 containing entire ΔL5tbpA (HindIII-XhoI) | This study |
| pVCU206 | pCR2.1 containing 5′ half of ΔL4tbpA (HindIII-BamHI) | This study |
| pVCU207 | pCR2.1 containing 3′ half of ΔL4tbpA (BamHI-XhoI) | This study |
| pVCU208 | pET21 containing entire ΔL4tbpA (HindIII-XhoI) | This study |
| pVCU209 | pCR2.1 containing 5′ half of ΔL8tbpA (HindIII-BamHI) | This study |
| pVCU210 | pCR2.1 containing 3′ half of ΔL8tbpA (BamHI-XhoI) | This study |
| pVCU211 | pET21 containing entire ΔL8tbpA (HindIII-XhoI) | This study |