TABLE 2.

Oligonucleotides used in this study

Name	Oligonucleotide sequencea	Amplification product
oVCU-16	5′-GGATCCGTTGTCCAAACCGATGCCCT-3′	ΔL4TbpA
oVCU-17	5′-GGATCCTTCGATACCGG2CAAAATCCG-3′	ΔL4TbpA
oUBAM	5′-AGGATCCCACGCCGCGCAGCATCAA-3′	ΔL5TbpA
oLBAM	5′-AGGATCCTGATGGCGGAGATTAGAGC-3′	ΔL5TbpA
oVCU-24	5′-GGATCCAATCAAATCGCGGTAGGCATT-3′	ΔL8TbpA
oVCU-25	5′-GGATCCGCCCAAAGCGCGCGGATT-3′	ΔL8TbpA
oVCU-7	5′-CTCGAGGCTCTAGAAACCCCAACGCAG-3′	All
HIND	5′-CGAAGAGTTGGGGCGGATGGTT-3′	All

^aMismatched bases, corresponding to mutagenic restriction sites, are shown in boldface. The superscript “2” indicates an error incorporated into the oligonucleotide.