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. 2022 Dec 16;12(12):2126. doi: 10.3390/life12122126

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Slight positivity for IL-8 in a few mononuclear cells, vehicle control group; (B) strong positivity for IL-8 in all mononuclear and lymphoid cells within the peribronchial area and the bronchial epithelial cells, positive control group; (C) strong positivity for IL-8 in all mononuclear, fibroblastic, endothelial cells, and bronchial epithelial cells, M1 trial group; (D) strong positivity for IL-8 in all mononuclear and lymphoid cells within the peribronchial area. Alveolar and bronchial epithelial cells were also intensely stained, YF1B-b trial group; (E) moderate positivity for IL-10 in the activated alveolar macrophages and PMNs, vehicle control group; (F) strong positivity for IL-10 in the peribronchial lymphoid and mononuclear cells, positive control group; (G) strong positivity for IL-10 in the peribronchial lymphoid and mononuclear cells, M1 trial group; (H) moderate positivity for IL-10 in PMNs and alveolar macrophages, YF1B-b group. Mayer Haematoxylin was used for background staining. Arrows show positive staining cells. (D) bar = 50 µm; in all other figures: bar = 20 µm.