Figure 1.
Overview of experimental protocols for behavioural and biochemical analysis in mice following Aβ1–42 i.c.v. infusion and guanosine treatment. Protocol 1: Mice were subjected to intracerebroventricular (i.c.v.) infusion of Aβ1–42 (400 pmol/3 uL site) or phosphate-buffered saline (PBS) infusion, and after 30 min received an intraperitoneal (i.p.) treatment of guanosine (GUO, 8 mg/kg) for 7 days. On the eighth day, mice were subjected to the open field test and to the tail suspension test, euthanized, and hippocampal slices assessed for ROS production and slice viability (MTT reduction and PI incorporation) (A). Protocol 2: On the tenth day after treatments, mice were subjected to the sucrose splash test and to the object location test. Animals were euthanized and hippocampal slices were assessed for ROS production, mitochondrial membrane potential, glutamate and lactate efflux, and slice viability (B).